Improved expression and purification of sigma 1 receptor fused to maltose binding protein by alteration of linker sequence

Sigma 1 receptor (S1R) is a eukaryotic membrane protein that functions as an inter-organelle signaling modulator and chaperone. Here we report an improved expression of S1R in Escherichia coli as a fusion to maltose binding protein (MBP) and a high-yield purification. Variants with linking amino aci...

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Detalhes bibliográficos
Main Authors: Gromek, Katarzyna A., Meddaugh, Hannah R., Wrobel, Russell L., Suchy, Fabian P., Bingman, Craig A., Primm, John G., Fox, Brian G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3679933/
https://ncbi.nlm.nih.gov/pubmed/23562661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2013.03.013
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