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In vitro transcription and delimitation of promoter elements of the murine dihydrofolate reductase gene.

We have developed an in vitro transcription system for the murine dihydrofolate reductase gene. Although transcription in vitro from a linearized template was initiated at the same start sites as in vivo, the correct ratios were more closely approximated when a supercoiled template was used. In addi...

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Detalhes bibliográficos
Main Authors: Farnham, P J, Schimke, R T
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC367792/
https://ncbi.nlm.nih.gov/pubmed/3785199
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