Purification of a cellular, double-stranded DNA-binding protein required for initiation of adenovirus DNA replication by using a rapid filter-binding assay.

A rapid and quantitative nitrocellulose filter-binding assay is described for the detection of nuclear factor I, a HeLa cell sequence-specific DNA-binding protein required for the initiation of adenovirus DNA replication. In this assay, the abundant nonspecific DNA-binding activity present in unfrac...

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Veröffentlicht in:	Mol Cell Biol
Hauptverfasser:	Diffley, J F, Stillman, B
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis 1986
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367659/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3785168/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.5.1363-1373.1986
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