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Large-scale production of polyoma middle T antigen by using genetically engineered tumors.

A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 t...

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Detalles Bibliográficos
Publicado en:Mol Cell Biol
Main Authors: Kaplan, D R, Bockus, B, Roberts, T M, Bolen, J, Israel, M, Schaffhausen, B S
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 1985
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367301/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2991752/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.7.1795
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