Large-scale production of polyoma middle T antigen by using genetically engineered tumors.

A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 t...

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Veröffentlicht in:	Mol Cell Biol
Hauptverfasser:	Kaplan, D R, Bockus, B, Roberts, T M, Bolen, J, Israel, M, Schaffhausen, B S
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis 1985
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367301/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2991752/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.7.1795-1799.1985
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