Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate.
The minimal substrate for the 2 microns circle site-specific recombinase FLP consists of a nearly perfect 13-base-pair dyad symmetry with an 8-base-pair core. By using a series of chemically synthesized FLP substrates in in vitro FLP recombination and FLP-binding assays, we have identified four posi...
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| Published in: | Mol Cell Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Taylor & Francis
1986
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367214/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3796604/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.12.4329-4334.1986 |
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