High-efficiency transformation of Saccharomyces cerevisiae cells by bacterial minicell protoplast fusion.

After a new transformation procedure, 10% of Saccharomyces cerevisiae cells were found to contain transforming DNA sequences. We used direct transfer of plasmid molecules by fusing bacterial minicell protoplasts to yeast protoplasts. Since the procedure significantly reduces the toxic effect of proc...

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Bibliographic Details
Main Authors: Gyuris, J, Duda, E G
Format: Artigo
Language:Inglês
Published: 1986
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC367070/
https://ncbi.nlm.nih.gov/pubmed/3023973
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