General method for cloning Neurospora crassa nuclear genes by complementation of mutants.

We have developed a sib selection procedure for cloning Neurospora crassa nuclear genes by complementation of mutants. This procedure takes advantage of a modified N. crassa transformation procedure that gives as many as 10,000 to 50,000 stable transformants per microgram of DNA with recombinant pla...

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Publié dans:	Mol Cell Biol
Auteurs principaux:	Akins, R A, Lambowitz, A M
Format:	Artigo
Langue:	Inglês
Publié:	Taylor & Francis 1985
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC366953/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2942762/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.9.2272-2278.1985
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