A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences

BACKGROUND: In vivo recombination of overlapping DNA fragments for assembly of large DNA constructs in the yeast Saccharomyces cerevisiae holds great potential for pathway engineering on a small laboratory scale as well as for automated high-throughput strain construction. However, the current in vi...

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Hlavní autoři: Kuijpers, Niels GA, Solis-Escalante, Daniel, Bosman, Lizanne, van den Broek, Marcel, Pronk, Jack T, Daran, Jean-Marc, Daran-Lapujade, Pascale
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2013
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3669052/
https://ncbi.nlm.nih.gov/pubmed/23663359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-12-47
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