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Biochemical and immunological characterization of mutant L-M cells with altered levels of dibutyryl cyclic AMP-inducible alkaline phosphatase.

Phosphotyrosine-Sepharose 4B was synthesized and used to purify L-cell alkaline phosphatase. Antibodies to this enzyme interacted with the alkaline phosphatase of strains A-1-2 and A-3-3, mutants that express the enzyme constitutively. This and thermal stability studies suggest that these mutants co...

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Bibliographic Details
Main Authors: Gum, J R, Raetz, C R
Format: Artigo
Language:Inglês
Published: 1985
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC366837/
https://ncbi.nlm.nih.gov/pubmed/2987678
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