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Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing

Transcript leaders (TLs) can have profound effects on mRNA translation and stability. To map TL boundaries genome-wide, we developed TL-sequencing (TL-seq), a technique combining enzymatic capture of m(7)G-capped mRNA 5′ ends with high-throughput sequencing. TL-seq identified mRNA start sites for th...

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Detalhes bibliográficos
Main Authors: Arribere, Joshua A., Gilbert, Wendy V.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3668365/
https://ncbi.nlm.nih.gov/pubmed/23580730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.150342.112
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