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Expression of the Escherichia coli dam methylase in Saccharomyces cerevisiae: effect of in vivo adenine methylation on genetic recombination and mutation.

The Escherichia coli DNA adenine methylase (dam) gene has been introduced into Saccharomyces cerevisiae on a yeast-E. coli shuttle vector. Sau3AI, MboI, and DpnI restriction enzyme digests and Southern hybridization analysis indicated that the dam gene is expressed in yeast cells and methylates GATC...

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Bibliografische gegevens
Gepubliceerd in:Mol Cell Biol
Hoofdauteurs: Hoekstra, M F, Malone, R E
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Taylor & Francis 1985
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC366761/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3887134/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.4.610-618.1985
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