Transfection of mouse fibroblast cells with a promoterless herpes simplex virus thymidine kinase gene: number of integrated gene copies and structure of single and amplified gene sequences.

Plasmids carrying the herpes simplex virus thymidine kinase (tk) gene were used to transfect thymidine kinase-deficient cells of the mouse fibroblast cell line LM(tk-). Individual cell clones were cultivated in selective hypoxanthine-aminopterin-thymidine medium to determine the number of integrated...

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Bibliographic Details
Main Authors: Pülm, W, Knippers, R
Format: Artigo
Language:Inglês
Published: 1985
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC366712/
https://ncbi.nlm.nih.gov/pubmed/2579320
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