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Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
Vacuolar and cytosolic pH are highly regulated in yeast cells and occupy a central role in overall pH homeostasis. We describe protocols for ratiometric measurement of pH in vivo using pH-sensitive fluorophores localized to the vacuole or cytosol. Vacuolar pH is measured using BCECF, which localizes...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
MyJove Corporation
2013
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3665305/ https://ncbi.nlm.nih.gov/pubmed/23629151 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50261 |
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