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A Novel Strategy for Cell-Autonomous Gene Knockdown in Caenorhabditis elegans Defines a Cell-Specific Function for the G-Protein Subunit GOA-1

We developed a novel knockdown strategy to examine cell-specific gene function in Caenorhabditis elegans. In this strategy a null mutation in any gene is replaced with a genetically stable transgene that contains a wild-type copy of the gene fused to a 3′ tag that targets the mRNA transcript for deg...

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Detalhes bibliográficos
Main Authors: Maher, Kathryn N., Swaminathan, Aishwarya, Patel, Parth, Chase, Daniel L.
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3664847/
https://ncbi.nlm.nih.gov/pubmed/23525334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.149724
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