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Improving accuracy of cell and chromophore concentration measurements using optical density

BACKGROUND: UV–vis spectrophotometric optical density (OD) is the most commonly-used technique for estimating chromophore formation and cell concentration in liquid culture. OD wavelength is often chosen with little thought given to its effect on the quality of the measurement. Analysis of the contr...

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Autori principali: Myers, John A, Curtis, Brandon S, Curtis, Wayne R
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3663833/
https://ncbi.nlm.nih.gov/pubmed/24499615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2046-1682-6-4
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