A standard vector for the chromosomal integration and characterization of BioBrick™ parts in Escherichia coli

BACKGROUND: The chromosomal integration of biological parts in the host genome enables the engineering of plasmid-free stable strains with single-copy insertions of the desired gene networks. Although different integrative vectors were proposed, no standard pre-assembled genetic tool is available to...

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Библиографические подробности
Главные авторы: Zucca, Susanna, Pasotti, Lorenzo, Politi, Nicolò, Cusella De Angelis, Maria Gabriella, Magni, Paolo
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2013
Предметы:
Research
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3662617/
https://ncbi.nlm.nih.gov/pubmed/23663425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-7-12
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