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A Robust Strategy for Negative Selection of Cre-LoxP Recombination-Based Excision of Transgenes in Induced Pluripotent Stem Cells

Viral vectors remain the most efficient and popular in deriving induced pluripotent stem cells (iPSCs). For translation, it is important to silence or remove the reprogramming factors after induction of pluripotency. In this study, we design an excisable loxP-flanked lentiviral construct that a) inc...

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Detalhes bibliográficos
Main Authors: Chakraborty, Syandan, Christoforou, Nicolas, Fattahi, Ali, Herzog, Roland W., Leong, Kam W.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3661507/
https://ncbi.nlm.nih.gov/pubmed/23717601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0064342
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