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Requirement for Extraction of Polyribosomes from Barley Tissue

The isolation of barley (Hordeum vulgare L.) polyribosomes, showing minimal degradation effects of endogenous RNase, required a buffer adjusted to pH 8.0 and containing 0.40 m KCl in addition to common extraction components. The extracted polyribosomes were characterized in sucrose gradients by thei...

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Detalhes bibliográficos
Main Authors: Breen, M. D., Whitehead, E. I., Kenefick, D. G.
Formato: Artigo
Idioma:Inglês
Publicado em: 1972
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC366043/
https://ncbi.nlm.nih.gov/pubmed/16658039
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