Defining ELISpot cut-offs from unreplicated test and control wells

In the absence of replication of wells, empirical criteria for enzyme-linked immunospot (ELISpot) positivity use fixed differences or ratios between spot forming units (SFU) counts between test and control. We propose an alternative approach which first identifies the optimally variance-stabilizing...

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Detalhes bibliográficos
Main Authors: Alexander, Neal, Fox, Annette, Lien, Vu Thi Kim, Dong, Tao, Lee, Laurel Yong-Hwa, Le Khanh Hang, Nguyen, Mai, Le Quynh, Horby, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2013
Assuntos:
Technical Note
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3657161/
https://ncbi.nlm.nih.gov/pubmed/23500146
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2013.02.014
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