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Cloning of the PYR3 gene of Ustilago maydis and its use in DNA transformation.
The Ustilago maydis PYR3 gene encoding dihydroorotase activity was cloned by direct complementation of Escherichia coli pyrC mutations. PYR3 transformants of E. coli pyrC mutants expressed homologous transcripts of a variety of sizes and regained dihydroorotase activity. PYR3 also complemented Sacch...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1988
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC365644/ https://ncbi.nlm.nih.gov/pubmed/2907604 |
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