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Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry

Single-cell mass spectrometry (MS) empowers metabolomic investigations by decreasing analytical dimensions to the size of individual cells and subcellular structures. We describe a protocol for investigating and quantifying metabolites in individual isolated neurons using single-cell capillary elect...

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Bibliographic Details
Main Authors: Nemes, Peter, Rubakhin, Stanislav S., Aerts, Jordan T., Sweedler, Jonathan V.
Format: Artigo
Language:Inglês
Published: 2013
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3655804/
https://ncbi.nlm.nih.gov/pubmed/23538882
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.035
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