Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae.

The isolation and characterization of temperature-sensitive mutations in RNA polymerase I from Saccharomyces cerevisiae are described. A plasmid carrying RPA190, the gene encoding the largest subunit of the enzyme, was subjected to in vitro mutagenesis with hydroxylamine. Using a plasmid shuffle scr...

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Publicado en:	Mol Cell Biol
Autores principales:	Wittekind, M, Dodd, J, Vu, L, Kolb, J M, Buhler, J M, Sentenac, A, Nomura, M
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Taylor & Francis 1988
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC365468/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3054507/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.10.3997-4008.1988
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