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High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays

We have described a protocol for performing high-throughput immunofluorescence microscopy on microarrays of yeast cells. This approach employs immunostaining of spheroplasted yeast cells printed as high-density cell microarrays, followed by imaging using automated microscopy. A yeast spheroplast mic...

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Detalles Bibliográficos
Autores principales: Niu, Wei, Hart, G. Traver, Marcotte, Edward M.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2011
Materias:
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3654672/
https://ncbi.nlm.nih.gov/pubmed/21104056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61737-970-3_7
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