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Intermolecular recombination assay for mammalian cells that produces recombinants carrying both homologous and nonhomologous junctions.

We present an intermolecular recombination assay for mammalian cells that does not involve the reconstitution of a selectable marker. It is based on the generation of a shuttle vector by recombination between a bacterial and a mammalian vector. The recombinants can thus be amplified in mammalian cel...

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Bibliographic Details
Main Authors: Brouillette, S, Chartrand, P
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC365349/
https://ncbi.nlm.nih.gov/pubmed/3037354
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