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Cloning of a human S-phase cell cycle gene: use of transient expression for screening.

We report here the cloning of a human cell cycle gene capable of complementing a temperature-sensitive (ts) S-phase cell cycle mutation in a Chinese hamster cell line. Cloning was performed as follows. A human genomic library in phage lambda containing 600,000 phages was screened with labeled cDNA s...

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Autores principales: Fainsod, A, Diamond, G, Marcus, M, Ruddle, F H
Formato: Artigo
Lenguaje:Inglês
Publicado: 1987
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC365134/
https://ncbi.nlm.nih.gov/pubmed/3029567
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