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Gateway Vectors for Efficient Artificial Gene Assembly In Vitro and Expression in Yeast Saccharomyces cerevisiae

Construction of synthetic genetic networks requires the assembly of DNA fragments encoding functional biological parts in a defined order. Yet this may become a time-consuming procedure. To address this technical bottleneck, we have created a series of Gateway shuttle vectors and an integration vect...

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Detalles Bibliográficos
Main Authors: Giuraniuc, Claudiu V., MacPherson, Murray, Saka, Yasushi
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2013
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3651225/
https://ncbi.nlm.nih.gov/pubmed/23675537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0064419
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