Elongation Factor Ts Directly Facilitates the Formation and Disassembly of the Escherichia coli Elongation Factor Tu·GTP·Aminoacyl-tRNA Ternary Complex

Aminoacyl-tRNA enters the translating ribosome in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here, we describe bulk steady state and pre-steady state fluorescence methods that enabled us to quantitatively explore the kinetic features of Escherichia coli ternary complex formation an...

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Detalhes bibliográficos
Main Authors: Burnett, Benjamin J., Altman, Roger B., Ferrao, Ryan, Alejo, Jose L., Kaur, Navdep, Kanji, Joshua, Blanchard, Scott C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2013
Assuntos:
Protein Synthesis and Degradation
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3650427/
https://ncbi.nlm.nih.gov/pubmed/23539628
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.460014
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