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Magnitude and Origin of the Enhanced Basicity of the Catalytic Glutamate of Triosephosphate Isomerase

Glu-167 of triosephosphate isomerase from Trypanosoma brucei brucei (TbbTIM) acts as the base to deprotonate substrate to form an enediolate phosphate trianion intermediate. We report that there is a large ~6 pK unit increase in the basicity of the carboxylate side chain of Glu-167 upon binding of t...

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Bibliographische Detailangaben
Hauptverfasser: Malabanan, M. Merced, Nitsch-Velasquez, Lucia, Amyes, Tina L., Richard, John P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3649849/
https://ncbi.nlm.nih.gov/pubmed/23560625
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja401504w
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