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Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Immunoelectron microscopy is a powerful tool to study biological molecules at the subcellular level. Antibodies coupled to electron-dense markers such as colloidal gold can reveal the localization and distribution of specific antigens in various tissues(1). The two most widely used techniques are pr...

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Autors principals: Zhong, Ling, Brown, Joshua C., Wells, Clive, Gerges, Nashaat Z.
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3643798/
https://ncbi.nlm.nih.gov/pubmed/23609099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50273
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