Stability of Drosophila RNA polymerase II elongation complexes in vitro.

We show that nuclear extract from Drosophila Kc cells supports efficient elongation by RNA polymerase II initiated from the actin 5C promoter. The addition of 0.3% Sarkosyl, 1 mg of heparin per ml, or 250 mM KCl immediately after initiation has two effects. First, the elongation rate is reduced 80 t...

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Veröffentlicht in:	Mol Cell Biol
Hauptverfasser:	Kephart, D D, Marshall, N F, Price, D H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC364378/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1373806/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.12.5.2067-2077.1992
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