Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD

The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the oligomeric state of the active helicase complex...

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Bibliographic Details
Main Authors: Chisty, Liisa T., Toseland, Christopher P., Fili, Natalia, Mashanov, Gregory I., Dillingham, Mark S., Molloy, Justin E., Webb, Martin R.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2013
Subjects:
Nucleic Acid Enzymes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3643603/
https://ncbi.nlm.nih.gov/pubmed/23535146
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt194
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