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Transformation of the fungus Absidia glauca by complementation of a methionine-auxotrophic strain affected in the homoserine-acetyltransferase gene

Transformation of fungi by complementation of auxotrophs is generally much more reliable than usage of antibiotic resistance markers. In order to establish such a system for the model zygomycete Absidia glauca, a stable methionine auxotrophic mutant was isolated after X-ray mutagenesis of the minus...

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Detalhes bibliográficos
Main Authors: Karimi, Sedighe, Wetzel, Jana, Wöstemeyer, Johannes, Burmester, Anke
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3642153/
https://ncbi.nlm.nih.gov/pubmed/23650600
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fob.2012.07.002
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