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Adaptive optics enables 3D STED microscopy in aberrating specimens

Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implem...

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Detalhes bibliográficos
Main Authors: Gould, Travis J., Burke, Daniel, Bewersdorf, Joerg, Booth, Martin J.
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635694/
https://ncbi.nlm.nih.gov/pubmed/23037223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.20.020998
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