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Extending the Resolution of the Light Microscope with 3D-SIM

As is well known, the resolution of the light microscope has been limited by diffraction to approximately 250 nm laterally and 600 nm axially. Furthermore, the contrast between focal planes (axial contrast) has been particularly limited due to the “missing cone” that results from physical limits on...

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Detalhes bibliográficos
Autor principal: Goodwin, Paul
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635420/
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