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Rapid and Efficient Methods for Preparing Globin- and rRNA-Depleted Directional RNA-Seq Libraries
Deep sequencing of cDNA prepared from total RNA (RNA-Seq) or mRNA (mRNA-Seq) has become the method of choice for transcript profiling, discovery of novel transcripts, and identification of alternative splicing events. However, standard whole-transcriptome approaches to RNA-Seq face a significant cha...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Association of Biomolecular Resource Facilities
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3635354/ |
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