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Cloning of methylated transforming DNA from Neurospora crassa in Escherichia coli.
An arg-2 mutant of Neurospora crassa was transformed to prototrophy with a pBR322-N. crassa genomic DNA library. Repeated attempts to recover the integrated transforming DNA or segments thereof by digestion, ligation, and transformation of Escherichia coli, with selection for the plasmid marker ampi...
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| Publicado no: | Mol Cell Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis
1988
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC363403/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2968501/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.5.2211 |
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