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Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.

The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were selected on the basis of resistance to paromo...

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Detalhes bibliográficos
Publicado no:Mol Cell Biol
Main Authors: Karrer, K M, Yao, M C
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC363326/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3380092/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.4.1664
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