Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells.
We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and ove...
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| Publicado no: | Mol Cell Biol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis
1988
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC363202/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3352604/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.2.764-769.1988 |
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