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A Novel Experimental Scheme to Fully Separate Permethylated Glycans and Allow Online RP-LC-MS with Simplified ESI-MS Analysis

Permethylation of glycans is one of the steps used in glycoprotein analysis. Typical LC-MS configurations involve reverse-phase chromatography coupled online to ESI-MS systems. Historically, reverse phase chromatography has been unsuccessful in separating permethylated glycans. The interactions betw...

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Detalhes bibliográficos
Main Authors: Tao, Shujuan, Orlando, Ron, Brown, Stacy, Hines, Jesse
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3630689/
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