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Two Methods for High-Throughput NGS Template Preparation for Small and Degraded Clinical Samples Without Automation

Clinical samples are difficult to prepare for NGS, because of the small amounts or degraded states of formalin-fixed tissue, plasma, urine, and single-cell DNA. Conventional whole genome amplification methods are too biased for NGS applications, and the existing NGS preparation kits require intermed...

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Detalhes bibliográficos
Main Authors: Kamberov, E., Tesmer, T., Mastronardi, M., Langmore, John
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3630563/
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