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The Comparison of Secretion and Export of hIGF-1 in Escherichia Coli Strain BL21 and DH5?

Heterologous Escherichia coli expression system was chosen and assayed for the synthesis of human insulin-like growth factor-l (hIGF-1) as a secreted fusion protein. hIGF-1 secretion was compared in two strains E.coli host (BL21 and DH5α) with the transformation of plasmid pEZZ18-hIGF1. A synthetic...

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Bibliographische Detailangaben
Hauptverfasser: Buddhachat, K., Ounjai, S., Chokepaichitkool, T., Kasekarn, W., Ongchai, S., Chomdej, S., Osathanunkul, Maslin
Format: Artigo
Sprache:Inglês
Veröffentlicht: Association of Biomolecular Resource Facilities 2012
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3630558/
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