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Whole-mount three-dimensional imaging of internally localized immunostained cells within mouse embryos

We describe a three-dimensional (3D) confocal imaging technique to characterize and enumerate rare, newly emerging hematopoietic cells located within the vasculature of whole-mount preparations of mouse embryos. However, the methodology is broadly applicable for examining the development and 3D arch...

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Autori principali: Yokomizo, Tomomasa, Yamada-Inagawa, Tomoko, Yzaguirre, Amanda D, Chen, Michael J, Speck, Nancy A, Dzierzak, Elaine
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3629302/
https://ncbi.nlm.nih.gov/pubmed/22322215
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.441
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