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High-resolution nucleosome mapping of targeted regions using BAC-based enrichment

We report a target enrichment method to map nucleosomes of large genomes at unprecedented coverage and resolution by deeply sequencing locus-specific mononucleosomal DNA enriched via hybridization with bacterial artificial chromosomes. We achieved ∼10 000-fold enrichment of specific loci, which enab...

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Autors principals: Yigit, Erbay, Zhang, Quanwei, Xi, Liqun, Grilley, Dan, Widom, Jonathan, Wang, Ji-Ping, Rao, Anjana, Pipkin, Matthew E.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3627574/
https://ncbi.nlm.nih.gov/pubmed/23413004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt081
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