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Targeted transformation of Ascobolus immersus and de novo methylation of the resulting duplicated DNA sequences.
To develop a method to modify genomic sequences in Ascobolus immersus by precisely reintroducing defined DNA segments previously manipulated in vitro, we investigated the effect of transforming DNA conformation on recombination with chromosomal sequences. Circular single-stranded DNA carrying the me...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1989
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC362747/ https://ncbi.nlm.nih.gov/pubmed/2674671 |
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