In vivo construction of linear vectors based on killer plasmids from Kluyveromyces lactis: selection of a nuclear gene results in attachment of telomeres.

Linear vectors based on plasmids pGKL1 and pGKL2 from Kluyveromyces lactis were obtained by in vivo recombination in Saccharomyces cerevisiae and selected for integration of the nuclear LEU2 gene. The linear hybrid molecules obtained had no proteins attached to their 5' ends, as is found for na...

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Bibliographic Details
Main Authors: Kämper, J, Meinhardt, F, Gunge, N, Esser, K
Format: Artigo
Language:Inglês
Published: 1989
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC362455/
https://ncbi.nlm.nih.gov/pubmed/2779572
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