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Use of Degradation Tags To Control Protein Levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803

We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steady-state fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP...

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Detalles Bibliográficos
Main Authors: Landry, Brian P., Stöckel, Jana, Pakrasi, Himadri B.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3623166/
https://ncbi.nlm.nih.gov/pubmed/23396339
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03741-12
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