Polyadenylation of mRNA: minimal substrates and a requirement for the 2' hydroxyl of the U in AAUAAA.

mRNA-specific polyadenylation can be assayed in vitro by using synthetic RNAs that end at or near the natural cleavage site. This reaction requires the highly conserved sequence AAUAAA. At least two distinct nuclear components, an AAUAAA specificity factor and poly(A) polymerase, are required to cat...

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Detalhes bibliográficos

Publicado no:	Mol Cell Biol
Principais autores:	Wigley, P L, Sheets, M D, Zarkower, D A, Whitmer, M E, Wickens, M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362276/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1969611/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.4.1705-1713.1990
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