Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro.
We have partially purified a poly(A) polymerase (PAP) from HeLa cell nuclear extract which is involved in the 3'-end formation of polyadenylated mRNA. PAP had a molecular weight of approximately 50 to 60 kilodaltons. In the presence of manganese ions, PAP was able to polyadenylate RNA nonspecificall...
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| Publié dans: | Mol Cell Biol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Taylor & Francis
1989
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362161/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2538718/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.1.193-203.1989 |
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