Exploring sRNA-mediated gene silencing mechanisms using artificial small RNAs derived from a natural RNA scaffold in Escherichia coli

An artificial small RNA (afsRNA) scaffold was designed from an Escherichia coli sRNA, SibC. Using the lacZ reporter system, the gene silencing effects of afsRNAs were examined to explore the sRNA-mediated gene-silencing mechanisms in E. coli. Substitution of the original target recognition sequence...

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Bibliografiske detaljer
Main Authors: Park, Hongmarn, Bak, Geunu, Kim, Sun Chang, Lee, Younghoon
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2013
Fag:
RNA
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3616725/
https://ncbi.nlm.nih.gov/pubmed/23393193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt061
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