Transcriptional activation in an improved whole-cell extract from Saccharomyces cerevisiae.

We report an improved in vitro transcription system for Saccharomyces cerevisiae. Small changes in assay and whole-cell extraction procedures increase selective initiation by RNA polymerase II up to 60-fold over previous conditions (M. Woontner and J. A. Jaehning, J. Biol. Chem. 265:8979-8982, 1990)...

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Bibliografski detalji
Glavni autori: Woontner, M, Wade, P A, Bonner, J, Jaehning, J A
Format: Artigo
Jezik:Inglês
Izdano: 1991
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC361333/
https://ncbi.nlm.nih.gov/pubmed/1875938
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